yihong ye Search Results


flag tagged atf4  (Addgene inc)
93
Addgene inc flag tagged atf4
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Flag Tagged Atf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/pm29895827-404-14-20?v=Addgene+inc
Average 93 stars, based on 1 article reviews
flag tagged atf4 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
yihong ye  (Addgene inc)
92
Addgene inc yihong ye
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Yihong Ye, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/pmc10013782__pnas__2207461120__sapp-121-5-7?v=Addgene+inc
Average 92 stars, based on 1 article reviews
yihong ye - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
rab9a mcherry  (Addgene inc)
93
Addgene inc rab9a mcherry
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Rab9a Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/pmc06974163-200-17-18?v=Addgene+inc
Average 93 stars, based on 1 article reviews
rab9a mcherry - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
prk5 flag usp13  (Addgene inc)
92
Addgene inc prk5 flag usp13
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Prk5 Flag Usp13, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/pmc07948425-190-21-7?v=Addgene+inc
Average 92 stars, based on 1 article reviews
prk5 flag usp13 - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
plasmids 78597  (Addgene inc)
93
Addgene inc plasmids 78597
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Plasmids 78597, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/pmc09842591-42-19-18?v=Addgene+inc
Average 93 stars, based on 1 article reviews
plasmids 78597 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
prk5 ha ufm1  (Addgene inc)
93
Addgene inc prk5 ha ufm1
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Prk5 Ha Ufm1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/bio_rxiv__2024__02__14__580383-231-4-10?v=Addgene+inc
Average 93 stars, based on 1 article reviews
prk5 ha ufm1 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
prk atf4 δc  (Addgene inc)
91
Addgene inc prk atf4 δc
Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and <t>ATF4</t> as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)
Prk Atf4 δc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yihong+ye/pmc09989924-119-30-42?v=Addgene+inc
Average 91 stars, based on 1 article reviews
prk atf4 δc - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier

Image Search Results


Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and ATF4 as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)

Journal: Nature communications

Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells.

doi: 10.1038/s41467-018-04602-0

Figure Lengend Snippet: Fig. 7 MTHFD2 is deregulated in cardiovascular disease. a Glycine to serine ratio in plasma of human subjects with no atherosclerotic plaque (NP) (n = 26), stable atherosclerotic plaque (SP) (n = 26), and unstable atherosclerotic plaque (UP) (n = 26) as assessed by mass spectrometry. b Scatter plots showing expression correlation in 126 human carotid plaque samples between MTHFD2 and genes of the MTHFD2 network (colored according to Fig. 3a) as well as Nrf2 (NFE2L2) and ATF4 as calculated by Pearson correlation. c, d Relative mRNA expression of MTHFD2 and SHMT2 in plaque material of human subjects with unstable atherosclerotic plaque (UP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8) (normalized to 18SrRNA) (n = 8). e, f Western blot analysis of MTHFD2 expression (e) and quantification (f) of plaque material from human subjects with unstable atherosclerotic plaque (SP) (n = 20), stable atherosclerotic plaque (SP) (n = 20), or non-atherosclerotic artery (NP) (n = 8). g–j Relative mRNA expression of Mthfd2, Phgdh, Shmt2, and Slc3a2 in mouse thoracic aortic rings kept in organ culture and exposed to medium (1% FCS) with or without oxPAPC and rapamycin as indicated for 8 h (normalized to 18SrRNA) (n ≥4). k, l Relative mRNA expression of Mthfd2 and Shmt2 in the endothelium of partially ligated left carotid artery (LCA) compared to healthy right carotid artery (RCA) 48 h post ligation (normalized to 18S rRNA) (n = 3). (*p ≤0.05 Student’s t test). m Relative mRNA expression of Mthfd2 in the endothelium of the left carotid artery of ApoE−/−mice which were fed with high fat diet (HFD) for 0, 1, or 4 days (normalized to 18S rRNA) (n = 5). n–q Relative mRNA expression of MTHFD2, PHGDH, CEBPB and PCK2 in HAEC exposed to HDL from healthy human subjects (n = 10) or human subjects with CAD (n = 10) for 4 h. (*p ≤0.05 Mann Whitney test). r, s Western blot analysis of MTHFD2 (r) and quantification (s) of HAEC treated as in f for 24 h (n = 10) (*p ≤0.05 Mann Whitney test). Data are represented as mean ± SEM, *p ≤0.05 (ANOVA with Newman−Keuls post-hoc test if not otherwise indicated)

Article Snippet: Plasmid overexpression in endothelial cells was performed with the Neon electroporation system (Invitrogen) with FLAG-tagged ATF4 (gift from Yihong Ye, Addgene plasmid pRK-ATF4 #26114)64.

Techniques: Clinical Proteomics, Mass Spectrometry, Expressing, Western Blot, Organ Culture, Ligation, MANN-WHITNEY